7,8-dihydroxyflavone-functionalized gold nanoparticles target the arginase enzyme of Leishmania donovani.
Pragya PrasannaPrakash KumarSaptarshi MandalTanvi PayalSaurabh KumarSk Ugir HossainProlay DasV RavichandiranDebabrata MandalPublished in: Nanomedicine (London, England) (2021)
Aim: To analyze the efficacy and possible mechanism of action of 7,8-dihydroxyflavone (DHF) and DHF synthesized gold nanoparticles (GNPs) against the parasite Leishmania donovani. Methods: GNPs were synthesized using DHF and characterized by dynamic light scattering, ζ potential, Fourier transform infrared spectroscopy, transmission electron microscopy and x-ray diffraction. The efficacy of DHF and DHF-GNP were tested against sensitive and drug-resistant parasites. GNP uptake was measured on macrophages by atomic absorption spectroscopy. Results: DHF and DHF-GNP (∼35 nm) were equally effective against sensitive and drug-resistant strains and inhibited the arginase activity of parasites. Increased IFN-γ and reduced IL-12 cytokine response showed a Th1/Th2-mediated cell death in macrophages. Conclusion: The low cytotoxicity and high biological activity of DHF-GNP may be useful for chemotherapy of leishmaniasis.
Keyphrases
- drug resistant
- gold nanoparticles
- electron microscopy
- multidrug resistant
- acinetobacter baumannii
- cell death
- high resolution
- escherichia coli
- plasmodium falciparum
- immune response
- photodynamic therapy
- dendritic cells
- reduced graphene oxide
- magnetic resonance
- magnetic resonance imaging
- quantum dots
- squamous cell carcinoma
- cell proliferation
- computed tomography
- cell cycle arrest