Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE.

Kevin Maafu JumaKenta MorimotoVishnu SharmaKirti SharmaRadhika BiyaniManish BiyaniTeisuke TakitaKiyoshi Yasukawa

Published in: Molecular biology reports (2024)

Our results demonstrate the capability of μTGGE for the rapid, precise, and cost-effective detection of SARS-CoV-2 spike protein D614 and G614 variants without the need for sequencing. Therefore, this approach holds considerable potential for use in point-of-care mutation assays for SARS-CoV-2 and other pathogens.

Keyphrases

sars cov
loop mediated isothermal amplification
respiratory syndrome coronavirus
real time pcr
label free
protein protein
amino acid
high throughput
copy number
sensitive detection
single cell
gram negative
climate change
human health
genome wide