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Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy.

Lloyd C MurfinMaria WeberSang Jun ParkWon Tae KimCarlos M Lopez-AlledClaire L McMullinFabienne Pradaux-CaggianoCatherine L LyallGabriele I Kociok-KöhnJannis WenkSteven D BullJuyoung YoonHwan Myung KimTony David JamesSimon E Lewis
Published in: Journal of the American Chemical Society (2019)
Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
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