Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy.
Lloyd C MurfinMaria WeberSang Jun ParkWon Tae KimCarlos M Lopez-AlledClaire L McMullinFabienne Pradaux-CaggianoCatherine L LyallGabriele I Kociok-KöhnJannis WenkSteven D BullJuyoung YoonHwan Myung KimTony David JamesSimon E LewisPublished in: Journal of the American Chemical Society (2019)
Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- high resolution
- reactive oxygen species
- label free
- high speed
- optical coherence tomography
- high throughput
- photodynamic therapy
- stem cells
- molecular docking
- mesenchymal stem cells
- mass spectrometry
- fluorescence imaging
- energy transfer
- real time pcr
- molecular dynamics simulations
- genetic diversity