New Nocobactin Derivatives with Antimuscarinic Activity, Terpenibactins A-C, Revealed by Genome Mining of Nocardia terpenica IFM 0406.
Julia ChenAndri FrediansyahDaniel MännleJan StraetenerHeike Brötz-OesterheltNadine ZiemertLeonard KaysserHarald GrossPublished in: Chembiochem : a European journal of chemical biology (2020)
We report a genomics-guided exploration of the metabolic potential of the brasilicardin producer strain Nocardia terpenica IFM 0406. Bioinformatics analysis of the whole genome sequence revealed the presence of a biosynthetic gene cluster presumably responsible for the generation of formerly unknown nocobactin derivatives. Mass spectrometry-assisted isolation led to the identification of three new siderophores, terpenibactins A (1), B (2) and C (3), which belong to the class of nocobactins. Their structures were elucidated by employing spectroscopic techniques. Compounds 1-3 demonstrated inhibitory activity towards the muscarinic M3 receptor, while exhibiting only a low cytotoxicity.
Keyphrases
- mass spectrometry
- single cell
- genome wide
- high resolution
- structure activity relationship
- molecular docking
- liquid chromatography
- copy number
- high performance liquid chromatography
- gas chromatography
- capillary electrophoresis
- human health
- risk assessment
- transcription factor
- amino acid
- dna methylation
- climate change