Oxidative stress-induced retinal damage is prevented by mild hypothermia in an ex vivo model of cultivated porcine retinas.
Ana M Mueller-BuehlHannah DoepperSven GrauthoffTobias KieblerLaura PetersJosé HurstSandra KuehnKarl U Bartz-SchmidtH Burkard DickStephanie Christine JoachimSven SchnichelsPublished in: Clinical & experimental ophthalmology (2020)
H2 O2 induced strong degenerative processes in porcine retinas. The role of oxidative stress in the progression of retinal diseases makes this ex vivo organ culture model suitable to investigate new therapeutic approaches. In the present study, the damaging effect of H2 O2 to several retinal cell types was counteracted or strongly alleviated through hypothermia treatment. Especially RGCs, which are affected in glaucoma disease, were protected due to a reduced apoptosis rate through hypothermia.
Keyphrases
- oxidative stress
- cardiac arrest
- optical coherence tomography
- optic nerve
- diabetic retinopathy
- diabetic rats
- brain injury
- ischemia reperfusion injury
- dna damage
- high glucose
- endoplasmic reticulum stress
- single cell
- induced apoptosis
- cell death
- stem cells
- mesenchymal stem cells
- heat stress
- stress induced
- smoking cessation