Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae.
K T MulroneyJ M HallX HuangE TurnbullN M BzdylA ChakeraU NaseerE M CoreaM J EllingtonK L HopkinsA L WesterO EkelundN WoodfordTimothy J J InglisPublished in: Scientific reports (2017)
The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- gram negative
- flow cytometry
- drug resistant
- acinetobacter baumannii
- escherichia coli
- single cell
- loop mediated isothermal amplification
- systematic review
- machine learning
- cell therapy
- deep learning
- high resolution
- stem cells
- cystic fibrosis
- electronic health record
- mass spectrometry
- diffusion weighted imaging
- molecularly imprinted
- replacement therapy
- solid phase extraction
- quantum dots
- smoking cessation
- liquid chromatography