Neurodegenerative diseases (NDDs) are mainly induced by oxidative stress which produces excessive reactive oxygen species (ROS). Quercetin (QU) is a potent antioxidant with some effects on NDDs. This study prepared and characterized a novel glucose-modified QU liposome (QU-Glu-Lip), aiming not only to overcome QU's poor water solubility and bioavailability but also to deliver more QU to brain tissue to enhance its neuroprotective effect. QU-Glu-Lip possessed encapsulation efficiency (EE) of 89.9%, homogenous particle sizes (116-124 nm), small PDI value (<0.3), zeta value -1.363 ± 0.437 mV, proper pH and salt stability, and proper cytotoxicity. The glucose-modified liposome penetrated the blood-brain barrier (BBB) mediated via the glucose transporter 1 (GLUT1) and was taken by neuronal cells more efficiently than liposome without glucose, according to bEnd.3 and PC12 cell tests. QU-Glu-Lip attenuated H 2 O 2 -induced oxidative damage to PC12 with higher cell viability (88.42%) and lower intracellular ROS compared to that of QU. QU-Glu-Lip had higher brain target ability and delivered more QU to neuronal cells, effectively exerting the antioxidative neuroprotection effect. There is potential for the QU-Glu-Lip application for more effective treatment of NDDs.
Keyphrases
- cerebral ischemia
- reactive oxygen species
- oxidative stress
- induced apoptosis
- blood glucose
- dna damage
- white matter
- brain injury
- anti inflammatory
- blood brain barrier
- resting state
- diabetic rats
- cell death
- cell cycle arrest
- type diabetes
- skeletal muscle
- physical activity
- body mass index
- bone marrow
- functional connectivity
- risk assessment
- stem cells
- climate change
- blood pressure
- adipose tissue
- weight gain
- ischemia reperfusion injury
- pi k akt
- high glucose
- smoking cessation
- drug induced