DNA from non-viable bacteria biases diversity estimates in the corals Acropora loripes and Pocillopora acuta.

Ashley M DunganLaura GeisslerAmanda S WilliamsCecilie Ravn GotzeEmily C FlynnLinda L BlackallMadeleine J H van Oppen

Published in: Environmental microbiome (2023)

Our study is highly relevant to microbiome studies on coral and other host organisms as it delivers a solution to excluding non-viable DNA in a complex community. These results provide novel insights into the dynamic nature of host-associated microbiomes and underline the importance of applying versatile tools in the analysis of metabarcoding or next-generation sequencing data sets.

Keyphrases

circulating tumor
cell free
single molecule
healthcare
mental health
electronic health record
big data
gene expression
copy number
data analysis