Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance.

Raquel Fonseca-MaldonadoLuana P MeleiroLuís F S MendesLuana F AlvesSibeli CarliLucas D MoreroLuis G M BassoAntonio J Costa-FilhoRichard John Ward

Published in: Biotechnology for biofuels (2017)

These results show that the non-specific binding of the BsCel5A-RtCBM11 to the lignin component of the cell wall is minimal, and represent the first reported use of EPR to directly study the interaction of glycoside hydrolyse enzymes with natural insoluble substrates.

Keyphrases

cell wall
dna binding
binding protein
ionic liquid
energy transfer
electron transfer