Detection of the Prostate Cancer Biomarker PCA3 with Electrochemical and Impedance-Based Biosensors.
Juliana Coatrini SoaresAndrey Coatrini SoaresValquiria da Cruz RodriguesMatias Eliseo MelendezAlexandre Cesar SantosEliney Ferreira FariaRui Manuel Vieira ReisAndre Lopes CarvalhoOsvaldo N OliveiraPublished in: ACS applied materials & interfaces (2019)
Diagnosis of prostate cancer via PCA3 biomarker detection is promising to be much more efficient than with the prostatic specific antigens currently used. In this study, we present the first electrochemical and impedance-based biosensors that are capable of detecting PCA3 down to 0.128 nmol/L. The biosensors were made with a layer of PCA3-complementary single-stranded DNA (ssDNA) probe, immobilized on a layer-by-layer (LbL) film of chitosan (CHT) and carbon nanotubes (MWCNT). They are highly selective to PCA3, which was confirmed in impedance measurements and with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). Using information visualization methods, we could also distinguish between cell lines expressing the endogenous PCA3 long noncoding RNA (lncRNA) from cells that did not contain detectable levels of this biomarker. Since the methods involved in fabrication the biosensors are potentially low cost, one may hope to deploy PCA3 tests in any laboratory of clinical analyses and even for point-of-care diagnostics.
Keyphrases
- prostate cancer
- label free
- long noncoding rna
- low cost
- radical prostatectomy
- carbon nanotubes
- gold nanoparticles
- ionic liquid
- drug delivery
- air pollution
- single molecule
- computed tomography
- loop mediated isothermal amplification
- signaling pathway
- dendritic cells
- molecularly imprinted
- real time pcr
- dual energy
- heavy metals
- cell free
- risk assessment
- immune response
- room temperature
- polycyclic aromatic hydrocarbons