Molecular diagnosis of Cytomegalovirus infection: clinical performance of the Aptima transcription-mediated amplification assay toward conventional qPCR chemistry on whole blood samples.
Paolo BottinoLisa PastroneElisa ZanottoFrancesca SidotiRossana CavalloCristina CostaPublished in: Journal of clinical microbiology (2024)
In this paper, we describe the clinical performance of a novel transcription-mediated amplification (TMA) assay for the detection and quantification of human Cytomegalovirus (HCMV) DNA from whole blood samples. This is a pivotal analysis in immunocompromised patients [transplanted, HIV-positive, and Hematopoietic Stem Cell (HSC) recipients], and molecular tests with high sensitivity and specificity are necessary to evaluate the HCMV viral load in these patients. To our knowledge, this is the first in-depth evaluation of TMA chemistry for HCMV diagnosis on whole blood samples. Moreover, also technical aspects of this assay make it suitable for clinical diagnostics.
Keyphrases
- end stage renal disease
- hiv positive
- ejection fraction
- newly diagnosed
- healthcare
- peritoneal dialysis
- men who have sex with men
- antiretroviral therapy
- south africa
- patient reported outcomes
- human immunodeficiency virus
- intensive care unit
- hiv infected
- circulating tumor cells
- sensitive detection
- circulating tumor
- extracorporeal membrane oxygenation