The first Japanese biobank of patient-derived pediatric acute lymphoblastic leukemia xenograft models.
Kuniaki TanakaItaru KatoYuu DobashiJun-Ichi ImaiTakashi MikamiHirohito KubotaHiroo UenoMamoru ItoSeishi OgawaTatsutoshi NakahataJunko TakitaHidemi ToyodaChitose OgawaSouichi AdachiShinya WatanabeHiroaki GotoPublished in: Cancer science (2022)
A lack of practical resources in Japan has limited preclinical discovery and testing of therapies for pediatric relapsed and refractory acute lymphoblastic leukemia (ALL), which has poor outcomes. Here, we established 57 patient-derived xenografts (PDXs) in NOD.Cg-Prkdc scid ll2rg tm1Sug /ShiJic (NOG) mice and created a biobank by preserving PDX cells including three extramedullary relapsed ALL PDXs. We demonstrated that our PDX mice and PDX cells mimicked the biological features of relapsed ALL and that PDX models reproduced treatment-mediated clonal selection. Our PDX biobank is a useful scientific resource for capturing drug sensitivity features of pediatric patients with ALL, providing an essential tool for the development of targeted therapies.
Keyphrases
- acute lymphoblastic leukemia
- induced apoptosis
- allogeneic hematopoietic stem cell transplantation
- cell cycle arrest
- acute myeloid leukemia
- hodgkin lymphoma
- diffuse large b cell lymphoma
- multiple myeloma
- high fat diet induced
- endoplasmic reticulum stress
- cell death
- small molecule
- oxidative stress
- signaling pathway
- metabolic syndrome
- cell therapy
- mesenchymal stem cells
- cell proliferation
- combination therapy
- replacement therapy
- childhood cancer