Pyrene-Labeled and Quaternized Chitosan: Synthesis, Characterization, and Its Potential Application for Fluorescently Trackable Nucleic Acid Delivery into Cells.
Kornkanya PratumyotPongsakorn YuntasiriPhim-On KhunsukTinnakorn PhuangkaewChutamath SittplangkoonThitiporn PattarakankulTanapat PalagaSuda KiatkamjornwongVoravee P HovenPublished in: Biomacromolecules (2023)
A chitosan derivative (Pyr-CS-HTAP) having pyrene (Pyr) and N -[(2-hydroxyl-3-trimethylammonium)] propyl (HTAP) units conjugated at C6 and C2 positions, respectively, was synthesized and characterized. Dynamic light scattering and scanning electron microscopy revealed that Pyr-CS-HTAP self-assembled into spherical nanoparticles with a hydrodynamic diameter of 211 ± 5 nm and a ζ-potential of +49 mV. The successful binding of Pyr-CS-HTAP with nucleic acid was ascertained by fluorescence resonance energy-transfer analysis and gel electrophoresis. Pyr-CS-HTAP facilitated the cellular uptake of nucleic acid up to 99%. Co-localization analysis using fluorescence microscopy revealed the endosomal escape of the Pyr-CS-HTAP/nucleic acid complexes and the successful release of the nucleic acid cargoes from the polyplexes into the nucleus. It is strongly believed that Pyr-CS-HTAP can potentially be developed into a fluorescently trackable gene delivery system in the future.
Keyphrases
- nucleic acid
- energy transfer
- electron microscopy
- single molecule
- quantum dots
- high resolution
- induced apoptosis
- photodynamic therapy
- single cell
- signaling pathway
- genome wide
- oxidative stress
- cell cycle arrest
- risk assessment
- current status
- high throughput
- copy number
- gene expression
- pet imaging
- optic nerve
- data analysis
- pet ct