Impact of Sample Preparation Methods on Single-Cell X-ray Microscopy and Light Elemental Analysis Evaluated by Combined Low Energy X-ray Fluorescence, STXM and AFM.
Lucia MerolleLorella PascoloLuisa ZupinPietro ParisseValentina BonanniGianluca GarianiTamara T LahDiana E BedollaSergio CrovellaGiuseppe RicciStefano IottiEmil MalucelliGeroge KourousiasAlessandra GianoncelliPublished in: Molecules (Basel, Switzerland) (2023)
Although cryofixation seems to be the most appropriate method as it allows for keeping cells closer to physiological conditions, it has some technical limitations. Paraformaldehyde, when used at the average concentration of 3.7%, is also an excellent alternative for X-ray microscopy.
Keyphrases
- high resolution
- single molecule
- high speed
- dual energy
- single cell
- high throughput
- induced apoptosis
- atomic force microscopy
- electron microscopy
- mass spectrometry
- cell cycle arrest
- rna seq
- computed tomography
- oxidative stress
- endoplasmic reticulum stress
- tandem mass spectrometry
- liquid chromatography
- simultaneous determination