Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS.
Nurdan Yazici BektaşEzgi ErsoyMehmet BogaTuğçe BoranErcan ÇınarGül ÖzhanAhmet Ceyhan GorenEsra Eroglu OzkanPublished in: Turkish journal of chemistry (2021)
The study aims to determine the secondary metabolites of Hypericum androsaemum L. extracts by liquid chromatography-high resolution mass spectrometry (LC-HRMS), and investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was evaluated by MTT assay, and apoptosis induction abilities on human prostate adenocarcinoma (PC-3), and hepatocellular carcinoma (Hep G2) cell lines. Accordingly, major secondary metabolites were found as hederagenin (762 ± 70.10 μg/g) in the leaves dichloromethane (LD), herniarin (167 ± 1.50 μg/g) in fruit dichloromethane (FD), (-)-epicatechin (6538 ± 235.36 μg/g) in the leaves methanol (LM), (-)-epigallocatechin gallate (758 ± 20.46 μg/g) in the fruit methanol (FM), and caffeic acid (370 ± 8.88 μg/g) in the fruit water (FW), and (3313 ± 79.51 μg/g) in the leaves water (LW) extracts. LM exerted strong antioxidant activity in DPPH free (IC50 10.94 ± 0.08 μg/mL), and ABTS cation radicals scavenging (IC50 9.09 ± 0.05 μg/mL) activities. FM exhibited cytotoxic activity with IC50 values of 73.23 ± 3.06 µg/mL and 31.64 ± 2.75 µg/mL on PC-3 and Hep G2 cell lines, respectively. Being the richest extract in terms of quillaic acid (630 ± 18.9 μg/g), which is a well-known cytotoxic triterpenoid with proven apoptosis induction ability on different cells, FM extract showed apoptosis induction activity with 64.75% on PC-3 cells at 50 μg/mL concentration. The study provides promising results about the potential of Hypericum androsaemum on cancer prevention.
Keyphrases
- high resolution mass spectrometry
- liquid chromatography
- oxidative stress
- cell cycle arrest
- cell death
- mass spectrometry
- endoplasmic reticulum stress
- prostate cancer
- induced apoptosis
- ms ms
- ultra high performance liquid chromatography
- tandem mass spectrometry
- squamous cell carcinoma
- simultaneous determination
- endothelial cells
- gas chromatography
- benign prostatic hyperplasia
- locally advanced
- papillary thyroid
- high resolution
- essential oil
- ionic liquid
- climate change
- single cell
- induced pluripotent stem cells
- squamous cell
- young adults