Antibiotic resistance and genetic diversity among Pseudomonas aeruginosa isolated from urinary tract infections in Iran.
Mohammad RahimzadehShahla ShahbaziSamira SabziMehri HabibiMohammad Reza Asadi KaramPublished in: Future microbiology (2023)
Aims: To determine the antibiotic resistance and genetic diversity of Pseudomonas aeruginosa isolates. Methods: The antibiotic resistance, genetic diversity and the conjugate transformation among Pseudomonas aeruginosa collected from patients with urinary tract infection in Tehran, Iran, was investigated. Results: Antibiotic resistance against cefepime was seen in 51.74% of the isolates, followed by amikacin (47.76%). bla OXA-10 and bla VIM were the most prevalent extended-spectrum β-lactamase and metallo-β-lactamases genes, respectively. Five clusters (C1-C5) were obtained by pulse field gel electrophoresis and multilocus sequence typing revealed two strain types, ST235 and ST664. Conjugation detected bla OXA-48 and bla NDM genes were transferred to Escherichia coli K12. Conclusion: The resistance of P. aeruginosa to antibiotics is increasing, which highlights the need to determine the resistance patterns to design better treatment strategies.
Keyphrases
- genetic diversity
- klebsiella pneumoniae
- urinary tract infection
- pseudomonas aeruginosa
- escherichia coli
- multidrug resistant
- cystic fibrosis
- biofilm formation
- acinetobacter baumannii
- genome wide
- gram negative
- drug resistant
- blood pressure
- bioinformatics analysis
- atomic force microscopy
- dna methylation
- genome wide analysis
- staphylococcus aureus