Despite the improvements in cancer treatment, breast cancer still remains the second most common cause of death from cancer in women. Doxorubicin (DOXO) is widely used for cancer treatment. However, drug resistance limits the treatment outcome. Here, we investigated the toxicity of DOXO in combination with an antifungal agent amphotericin B (AmB) against the MCF-7 breast cancer cell line. The cell viability was measured using MTT assay. The apoptosis was studied by caspase-8 and caspase-9 activity measurements and DNA fragmentation was investigated by TUNEL assay. The combination of two drugs significantly increased the apoptotic index and the caspase-8 and caspase-9 activities in comparison to DOXO-treated cells. Our finding showed that pre-treatment of MCF-7 cells with AmB synergistically exerted the anticancer effect of DOXO through the caspase-dependent apoptosis manner.
Keyphrases
- cell death
- cell cycle arrest
- induced apoptosis
- breast cancer cells
- endoplasmic reticulum stress
- oxidative stress
- signaling pathway
- drug delivery
- polycystic ovary syndrome
- cancer therapy
- papillary thyroid
- squamous cell carcinoma
- atomic force microscopy
- pregnancy outcomes
- adipose tissue
- type diabetes
- drug induced
- mass spectrometry
- high resolution
- newly diagnosed
- childhood cancer
- insulin resistance
- single cell
- cell proliferation