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In Situ Stability Test for mRNA Vaccines Based on Deep-UV Resonance Raman Spectroscopy.

Lamyaa M AlmehmadiSergei V ReverdattoVladimir V ErmolenkovAlexander ShekhtmanIgor K Lednev
Published in: Analytical chemistry (2023)
Deep-UV resonance Raman spectroscopy has been shown to offer great potential for probing the in situ stability of mRNA vaccines. In this study, a vaccine model was subjected to controlled degradation using RNase A or through aging at room temperature. The degradation of mRNA was confirmed by using a cell transfection test and by gel electrophoresis. Under both settings, DUVRR spectroscopy successfully revealed the mRNA degradation signs of the vaccine model.
Keyphrases
  • raman spectroscopy
  • room temperature
  • binding protein
  • single cell
  • energy transfer
  • single molecule
  • cell therapy
  • stem cells
  • risk assessment
  • mass spectrometry
  • mesenchymal stem cells
  • quantum dots
  • wound healing