Quantifying Nanoparticle Layer Topography: Theoretical Modeling and Atomic Force Microscopy Investigations.
Zbigniew AdamczykMarta SadowskaMałgorzata Nattich-RakPublished in: Langmuir : the ACS journal of surfaces and colloids (2023)
A comprehensive method consisting of theoretical modeling and experimental atomic force microscopy (AFM) measurements was developed for the quantitative analysis of nanoparticle layer topography. Analytical results were derived for particles of various shapes such as cylinders (rods), disks, ellipsoids, hemispheres (caps), etc. It was shown that for all particles, their root-mean-square ( rms ) parameter exhibited a maximum at the coverage about 0.5, whereas the skewness was a monotonically decreasing function of the coverage. This enabled a facile determination of the particle coverage in the layer, even if the shape and size were not known. The validity of the analytical results was confirmed by computer modeling and experimental data acquired by AFM measurements for polymer nanoparticle deposition on mica and silica. The topographical analysis developed in this work can be exploited for a quantitative characterization of self-assembled layers of nano- and bioparticles, e.g., carbon nanotubes, silica and noble metal particles, DNA fragments, proteins, vesicles, viruses, and bacteria at solid surfaces. The acquired results also enabled a proper calibration, in particular the determination of the measurement precision, of various electron and scanning probe microscopies, such as AFM.
Keyphrases
- atomic force microscopy
- high speed
- single molecule
- carbon nanotubes
- high resolution
- affordable care act
- iron oxide
- solid phase extraction
- living cells
- quantum dots
- liquid chromatography
- molecularly imprinted
- electronic health record
- circulating tumor
- health insurance
- deep learning
- healthcare
- cell free
- mass spectrometry
- escherichia coli
- solar cells
- cystic fibrosis
- simultaneous determination
- low cost
- candida albicans