Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens).
Zhenghui GaoWenhui DengFen ZhuPublished in: PloS one (2019)
Hermetia illucens is an important resource insect for the conversion of organic waste. Quantitative PCR (qPCR) is the primary tool of gene expression analysis and a core technology of molecular biology research. Reference genes are essential for qPCR analysis; however, a stability analysis of H. illucens reference genes has not yet been carried out. To find suitable reference genes for normalizing gene expression data, the stability of eight housekeeping genes (including ATP6V1A, RPL8, EF1, Tubulin, TBP, GAPDH, Actin and RP49) was investigated under both biotic (developmental stages, tissues and sex) and abiotic (heavy metals, food, antibiotics) conditions. Gene expression data were analysed by geNorm, NormFinder, BestKeeper, and ΔCt programs. A set of specific reference genes was recommended for each experimental condition using the results of RefFinder synthesis analysis. This study offers a solid foundation for further studies of the molecular biology of H. illucens.
Keyphrases
- genome wide identification
- gene expression
- genome wide
- dna methylation
- heavy metals
- transcription factor
- genome wide analysis
- bioinformatics analysis
- high resolution
- magnetic resonance imaging
- risk assessment
- computed tomography
- public health
- climate change
- machine learning
- health risk
- artificial intelligence
- drinking water
- magnetic resonance
- atomic force microscopy
- real time pcr
- single molecule
- drosophila melanogaster