Transinteractome analysis reveals distinct niche requirements for isotype-based plasma cell subsets in the bone marrow.
Amélie BonaudPierre LarraufieMélanie KhamyathUgo SzachnowskiShaun M FlintNadège Brunel-MeunierFrançois DelhommeauAnnie MunierTapio LönnbergClaire Toffano-NiocheDaniel GautheretKarl BalabanianMarion EspéliPublished in: European journal of immunology (2023)
Bone marrow (BM) long-lived plasma cells (PCs) are essential for long-term protection against infection, and their persistence within this organ relies on interactions with Cxcl12-expressing stromal cells that are still not clearly identified. Here, using single cell RNAseq and in silico transinteractome analyses, we identified Leptin receptor positive (LepR + ) mesenchymal cells as the stromal cell subset most likely to interact with PCs within the BM. Moreover, we demonstrated that depending on the isotype they express, PCs may use different sets of integrins and adhesion molecules to interact with these stromal cells. Altogether, our results constitute an unprecedented characterization of PC subset stromal niches and open new avenues for the specific targeting of BM PCs based on their isotype.
Keyphrases
- bone marrow
- single cell
- induced apoptosis
- cell cycle arrest
- mesenchymal stem cells
- rna seq
- cell therapy
- stem cells
- cancer therapy
- cell death
- high throughput
- oxidative stress
- endoplasmic reticulum stress
- escherichia coli
- peripheral blood
- cell proliferation
- pi k akt
- pseudomonas aeruginosa
- cystic fibrosis
- candida albicans
- cell adhesion
- cell migration