Stepwise development of a homozygous S80P substitution in Fks1p, conferring echinocandin resistance in Candida tropicalis.
Rasmus Krøger HareHelle Krogh JohansenMaiken Cavling ArendrupPublished in: Antimicrobial agents and chemotherapy (2012)
Three Candida tropicalis isolates were obtained from a patient with acute lymphoblastic leukemia. The first isolate was susceptible to all drug classes, while isolates 2 and 3, obtained after 8 and 8.5 weeks of caspofungin treatment, respectively, were resistant to the three echinocandins. Multilocus sequence genotyping suggested a clonal relation among all isolates. FKS1 sequencing revealed a stepwise development of a heterozygous and finally a homozygous mutation, leading to S80S/P and S80P amino acid substitutions.
Keyphrases
- genetic diversity
- acute lymphoblastic leukemia
- amino acid
- candida albicans
- single cell
- biofilm formation
- early onset
- high throughput
- emergency department
- allogeneic hematopoietic stem cell transplantation
- pseudomonas aeruginosa
- gene expression
- staphylococcus aureus
- escherichia coli
- dna methylation
- acute myeloid leukemia
- drug induced
- smoking cessation
- preterm birth