Adipose triglyceride lipase suppresses noncanonical inflammasome by hydrolyzing LPS.
Weitao LiQiannv LiuYan QianChunlei WangChun KongLiangliang SunLi SunHongwei LiuYan ZhangDong JiangChangtao JiangShuo WangPengyan XiaPublished in: Nature chemical biology (2024)
Intracellular recognition of lipopolysaccharide (LPS) by mouse caspase-11 or human caspase-4 is a vital event for the activation of the noncanonical inflammasome. Whether negative regulators are involved in intracellular LPS sensing is still elusive. Here we show that adipose triglyceride lipase (ATGL) is a negative regulator of the noncanonical inflammasome. Through screening for genes participating in the noncanonical inflammasome, ATGL is identified as a negative player for intracellular LPS signaling. ATGL binds LPS and catalyzes the removal of the acylated side chains that contain ester bonds. LPS with under-acylated side chains no longer activates the inflammatory caspases. Cells with ATGL deficiency exhibit enhanced immune responses when encountering intracellular LPS, including an elevated secretion of interleukin-1β, decreased cell viability and increased cell cytotoxicity. Moreover, ATGL-deficient mice show exacerbated responses to endotoxin challenges. Our results uncover that ATGL degrades cytosolic LPS to suppress noncanonical inflammasome activation.
Keyphrases
- inflammatory response
- anti inflammatory
- induced apoptosis
- immune response
- toll like receptor
- adipose tissue
- cell death
- reactive oxygen species
- oxidative stress
- insulin resistance
- signaling pathway
- dna methylation
- single cell
- genome wide
- gene expression
- cell therapy
- smoking cessation
- cell cycle arrest
- induced pluripotent stem cells