Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories.
Kathy L Toohey-KurthDonna M MulrooneySusanne HinkleyMary Lea KillianJanice C PedersenMangkey A BounphengRoman PogranichniySteve BolinRoger MaesRebecca L TallmadgeLaura B GoodmanBeate M CrossleyPublished in: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (2020)
The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature.