Intravital microscopy visualizes innate immune crosstalk and function in tissue microenvironment.

Significant advances have been made in the field of intravital microscopy (IVM) on myeloid cells due to the growing number of validated fluorescent probes and reporter mice. IVM provides a visualization platform to directly observe cell behavior and deepen our understanding of cellular dynamics, heterogeneity, plasticity and cell-cell communication in native tissue environment. This review outlines the current studies on the dynamic interaction and function of innate immune cells with a focus on those that are studied with IVM and covers the advances in data analysis with emerging artificial intelligence-based algorithms. Finally, the future prospects of IVM on innate immune cells are discussed. This article is protected by copyright. All rights reserved.