Based on morphological and molecular evidence of five male adult specimens collected from Napo County, Baise City, Guangxi Zhuang Autonomous Region, China, we describe a new species of Rhacophorus , Rhacophorusnapoensis sp. nov. This new species is similar to Rhacophorusrhodopus Liu & Hu, 1959 and Rhacophorusbipunctatus Ahl, 1927 in morphology, but it can be distinguished from the latter two by the following morphological characteristics: head width is greater than head length, snout pointed, loreal region oblique, tympanum distinct, maxillary teeth distinct, tongue cordiform, external single subgular vocal sac, tibiotarsal articulation reaches the snout, tibia length is greater than foot length and slightly greater than half of snout-vent length, and single outer metatarsal tubercle is flat. The phylogenetic tree constructed based on 16S rRNA sequence shows that all individuals of this species clustered into the same clade, and genetically this new species differs from R.rhodopus and R.bipunctatus by 7.71% and 7.98% in 16S rRNA sequences, respectively.
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