An adaptive method of defining negative mutation status for multi-sample comparison using next-generation sequencing.
Nicholas HutsonFenglin ZhanJames GrahamMitsuko MurakamiHan ZhangSujana GanapartiQiang HuLi YanChangxing MaSong LiuJun XieLei WeiPublished in: BMC medical genomics (2021)
We developed a new adaptive method for distinguishing unknown from negative statuses in multi-sample comparison NGS data. The method can provide more accurate negative statuses than the conventional UMC method and generate a remarkably higher amount of available data by reducing unnecessary "unknown" calls.