Single-cell proteomics enabled by next-generation sequencing or mass spectrometry.
Hayley M BennettWilliam StephensonChristopher M RoseSpyros DarmanisPublished in: Nature methods (2023)
In the last decade, single-cell RNA sequencing routinely performed on large numbers of single cells has greatly advanced our understanding of the underlying heterogeneity of complex biological systems. Technological advances have also enabled protein measurements, further contributing to the elucidation of cell types and states present in complex tissues. Recently, there have been independent advances in mass spectrometric techniques bringing us one step closer to characterizing single-cell proteomes. Here we discuss the challenges of detecting proteins in single cells by both mass spectrometry and sequencing-based methods. We review the state of the art for these techniques and propose that there is a space for technological advancements and complementary approaches that maximize the advantages of both classes of technologies.
Keyphrases
- single cell
- mass spectrometry
- rna seq
- induced apoptosis
- high throughput
- cell cycle arrest
- liquid chromatography
- high resolution
- gene expression
- gas chromatography
- high performance liquid chromatography
- endoplasmic reticulum stress
- cell death
- signaling pathway
- stem cells
- oxidative stress
- ms ms
- dna methylation
- protein protein
- bone marrow
- cell proliferation
- amino acid
- tandem mass spectrometry