A new α-pyrone from Arthrinium pseudosinense culture medium and its estrogenic activity in MCF-7 cells.
Haeun KwonQuynh Nhu NguyenMyung Woo NaKi Hyun KimYuanqiang GuoJoung Han YimSang Hee ShimJae-Jin KimKi Sung KangDongho LeePublished in: The Journal of antibiotics (2021)
A new α-pyrone analog, arthrifuranone A (1) was isolated from an EtOAc-extract of Arthrinium pseudosinense culture medium. The isolation workflow was guided by a Molecular Networking-based dereplication strategy. The chemical structure of the new compound was elucidated using MS and NMR spectroscopic techniques, and the absolute configuration was established by the Mosher's method and gauge-including atomic orbital NMR chemical shift calculations, followed by DP4 + analysis. The isolated compound was evaluated for its estrogenic activity using the MCF-7 estrogen responsive human breast cancer cells. Compound 1 showed estrogenic activity by increasing the proliferation of MCF-7 cells at the concentration of 3.125 μM via phosphorylation of estrogen receptor-α.
Keyphrases
- breast cancer cells
- estrogen receptor
- induced apoptosis
- cell cycle arrest
- magnetic resonance
- high resolution
- signaling pathway
- endothelial cells
- oxidative stress
- multiple sclerosis
- solid state
- endoplasmic reticulum stress
- molecular docking
- cell death
- molecular dynamics simulations
- molecular dynamics
- density functional theory
- protein kinase
- anti inflammatory