Bacterial production and biophysical characterization of a hard-to-fold scFv against myeloid leukemia cell surface marker, IL-1RAP.

Aref Farokhi-FardElham BayatArezoo Beig ParikhaniSamira Komijani Hooman Aghamirza Moghim Aliabadi Soroush SardariBehrouz GharibFarzaneh Barkhordari Kayhan Azadmanesh Morteza Karimipoor Haleh Bakhshandeh Fatemeh Davami

Published in: Molecular biology reports (2022)

We were able to produce 21 mg/L culture functional and stable anti-IL-1RAP scFv via recovering IBs by pulse dilution procedure. The produced scFv as a useful targeting agent could be used in scheming new therapeutics or diagnostics for myeloid malignancies.

Keyphrases

cell surface
bone marrow
acute myeloid leukemia
dendritic cells
blood pressure
small molecule
cancer therapy
irritable bowel syndrome
mass spectrometry
immune response