A Single-Component Luminescent Biosensor for the SARS-CoV-2 Spike Protein.
Matthew RavalinHeegwang RohRahul SuryawanshiG Renuka KumarJohn E PakMelanie OttAlice Y TingPublished in: Journal of the American Chemical Society (2022)
Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of Escherichia coli and Saccharomyces cerevisiae . This biosensor is deployed in multiple homogeneous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an unaugmented cell phone camera. Binding-activated tandem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest.
Keyphrases
- sars cov
- label free
- quantum dots
- sensitive detection
- loop mediated isothermal amplification
- escherichia coli
- saccharomyces cerevisiae
- respiratory syndrome coronavirus
- gold nanoparticles
- real time pcr
- protein protein
- endothelial cells
- high throughput
- stem cells
- small molecule
- ionic liquid
- immune response
- single cell
- deep learning
- pseudomonas aeruginosa
- multidrug resistant
- staphylococcus aureus