Conversion of Synthetic Aβ to In Vivo Active Seeds and Amyloid Plaque Formation in a Hippocampal Slice Culture Model.
Renata NovotnyFranziska LangerJasmin MahlerAngelos SkodrasAndreas VlachosBettina M Wegenast-BraunStephan A KaeserJonas J NeherYvonne S EiseleMarie J PietrowskiK Peter R NilssonThomas DellerMatthias StaufenbielBernd HeimrichMathias JuckerPublished in: The Journal of neuroscience : the official journal of the Society for Neuroscience (2017)
In this study, we report the seeded induction of Aβ aggregation and deposition in long-term hippocampal slice cultures. Remarkably, we find that the biological activities of the largely synthetic Aβ aggregates in the culture are very similar to those observed in vivo This observation is the first to show that potent in vivo seeding-active Aβ aggregates can be obtained by seeded conversion of synthetic Aβ in a living (wild-type) cellular environment.