Genome structural dynamics: insights from Gaussian network analysis of Hi-C data.
Anupam BanerjeeShe ZhangIvet BaharPublished in: Briefings in functional genomics (2024)
Characterization of the spatiotemporal properties of the chromatin is essential to gaining insights into the physical bases of gene co-expression, transcriptional regulation and epigenetic modifications. The Gaussian network model (GNM) has proven in recent work to serve as a useful tool for modeling chromatin structural dynamics, using as input high-throughput chromosome conformation capture data. We focus here on the exploration of the collective dynamics of chromosomal structures at hierarchical levels of resolution, from single gene loci to topologically associating domains or entire chromosomes. The GNM permits us to identify long-range interactions between gene loci, shedding light on the role of cross-correlations between distal regions of the chromosomes in regulating gene expression. Notably, GNM analysis performed across diverse cell lines highlights the conservation of the global/cooperative movements of the chromatin across different types of cells. Variations driven by localized couplings between genomic loci, on the other hand, underlie cell differentiation, underscoring the significance of the four-dimensional properties of the genome in defining cellular identity. Finally, we demonstrate the close relation between the cell type-dependent mobility profiles of gene loci and their gene expression patterns, providing a clear demonstration of the role of chromosomal 4D features in defining cell-specific differential expression of genes.
Keyphrases
- genome wide
- copy number
- dna methylation
- gene expression
- network analysis
- high throughput
- induced apoptosis
- electronic health record
- single cell
- physical activity
- stem cells
- transcription factor
- machine learning
- high resolution
- big data
- endoplasmic reticulum stress
- signaling pathway
- minimally invasive
- bone marrow
- mass spectrometry
- long non coding rna
- genome wide association study
- cell proliferation
- cell cycle arrest
- artificial intelligence