Impact of sequencing depth and technology on de novo RNA-Seq assembly.
Jordan PattersonEric J CarpenterZhenzhen ZhuDan AnXinming LiangChunyu GengRadoje DrmanacGane Ka-Shu WongPublished in: BMC genomics (2019)
Increasing sequencing depth beyond modest data sets of less than 10 Gbp recovers a plethora of single-exon transcripts undocumented in genome annotations. DNBseq™ is a viable alternative to HiSeq for de novo RNA-Seq assembly.