Method development and validation for analysis of microencapsulated cyanidin-3-O-rutinoside in dairy samples containing Juçara palm fruit by HPLC-PDA.

Euterpe edulis is a plant native of Brazil and popularly known as Juçara. The aim of this study was to develop and validate an analytical method by HPLC of the Juçara crude extracts (JCE), and to evaluate dairy products containing free and microencapsulated extracts. The validated method of the JCE was performed, using cyanidin-3-O-rutinoside (C3R), isolated and identified as major component from JCE, as reference: linearity was used 10 concentrations between 25 and 250 μg mL -1 (R 2 =0.994); LOD and LOQ: 19.04 and 57.7 μg mL -1 , respectively; accuracy RSD%<5; recovery, 104.85 μg mL -1 , and robustness. Physicochemical analyses of the dairy product were evaluated by colour, pH, and concentration of anthocyanins by HPLC calculated as C3R over 21 days of storage. The pH remained stable until the end of the test. The colouring and the concentration of anthocyanins remained stable for nine days. It was isolated C3R, the HPLC method was developed and validated, and colour stability was not altered until nine days. This article is protected by copyright. All rights reserved.