A high-performance electrochemical biosensor using an engineered urate oxidase.

Zheng Wei Tanja Knaus Yuxin LiuZiran ZhaiAndrea F G Gargano Gadi Rothenberg Ning Yan Francesco G Mutti

Published in: Chemical communications (Cambridge, England) (2023)

We constructed a high-performance biosensor for detecting uric acid by immobilizing an engineered urate oxidase on gold nanoparticles deposited on a carbon-glass electrode. This biosensor showed a low limit-of-detection (9.16 nM), a high sensitivity (14 μA/μM), a wide range of linearity (50 nM-1 mM), and more than 28 days lifetime.

Keyphrases

gold nanoparticles
uric acid
label free
metabolic syndrome
reduced graphene oxide
photodynamic therapy
sensitive detection
quantum dots
wastewater treatment
mass spectrometry
high resolution
carbon nanotubes