Genetic Variations miR-10a A>T, miR-30c A>G, miR-181a T>C, and miR-499b A>G and the Risk of Recurrent Pregnancy Loss in Korean Women.

This study investigated the genetic association between recurrent pregnancy loss (RPL) and microRNA (miRNA) polymorphisms in miR-10a A>T, miR-30c A>G, miR-181a T>C, and miR-499b A>G in Korean women. Blood samples were collected from 381 RPL patients and 281 control participants, and genotyping of miR-10a A>T, miR-30c A>G, miR-181a T>C, and miR-499b A>G was carried out by TaqMan miRNA RT-Real Time polymerase chain reaction (PCR). Four polymorphisms were identified, including miR-10a A>T, miR-30c A>G, miR-181a T>C, and miR-499b A>G. MiR-10a dominant model (AA vs. AT + TT) and miR-499b GG genotypes were associated with increased RPL risk (adjusted odds ratio [AOR] = 1.520, 95% confidence interval [CI] = 1.038-2.227, p = 0.032; AOR = 2.956, 95% CI = 1.168-7.482, p = 0.022, respectively). Additionally, both miR-499 dominant (AA vs. AG + GG) and recessive (AA + AG vs. GG) models were significantly associated with increased RPL risk (AOR = 1.465, 95% CI = 1.062-2.020, p = 0.020; AOR = 2.677, 95% CI = 1.066-6.725, p = 0.036, respectively). We further propose that miR-10a A>T, miR-30c A>G, and miR-499b A>G polymorphisms effects could contribute to RPL and should be considered during RPL patient evaluation.