Behavior of Calcium Phosphate-Chitosan-Collagen Composite Coating on AISI 304 for Orthopedic Applications.
Claudio ZancaBernardo PatellaElisa CapuanaFrancesco LoprestiValerio Maria Bartolo BrucatoFrancesco Carfì PaviaVincenzo La CarrubbaRosalinda InguantaPublished in: Polymers (2022)
Calcium phosphate/chitosan/collagen composite coating on AISI 304 stainless steel was investigated. Coatings were realized by galvanic coupling that occurs without an external power supply because it begins with the coupling between two metals with different standard electrochemical potentials. The process consists of the co-deposition of the three components with the calcium phosphate crystals incorporated into the polymeric composite of chitosan and collagen. Physical-chemical characterizations of the samples were executed to evaluate morphology and chemical composition. Morphological analyses have shown that the surface of the stainless steel is covered by the deposit, which has a very rough surface. XRD, Raman, and FTIR characterizations highlighted the presence of both calcium phosphate compounds and polymers. The coatings undergo a profound variation after aging in simulated body fluid, both in terms of composition and structure. The tests, carried out in simulated body fluid to scrutinize the corrosion resistance, have shown the protective behavior of the coating. In particular, the corrosion potential moved toward higher values with respect to uncoated steel, while the corrosion current density decreased. This good behavior was further confirmed by the very low quantification of the metal ions (practically absent) released in simulated body fluid during aging. Cytotoxicity tests using a pre-osteoblasts MC3T3-E1 cell line were also performed that attest the biocompatibility of the coating.
Keyphrases
- wound healing
- drug delivery
- room temperature
- tissue engineering
- hyaluronic acid
- cancer therapy
- physical activity
- gold nanoparticles
- mental health
- intellectual disability
- human health
- ionic liquid
- label free
- climate change
- autism spectrum disorder
- molecularly imprinted
- electron transfer
- health risk
- single molecule
- high resolution