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A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo.

Yuyao LiHeng SongChenghong XueZhijun FangLiqin XiongHexin Xie
Published in: Chemical science (2020)
Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity in vivo. Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic in vivo system.
Keyphrases
  • living cells
  • quantum dots
  • fluorescent probe
  • single molecule
  • high resolution
  • deep learning
  • hydrogen peroxide
  • nitric oxide
  • mass spectrometry
  • sensitive detection
  • fluorescence imaging