Facile synthesis of hollow microtubular COF as enrichment probe for quantitative detection of ultratrace quinones in mice plasma with APGC-MS/MS.

A hollow microtubular covalent organic framework (denoted as TatDha-COF) was synthesized by solvothermal method for the enrichment and determination of quinones. The TatDha-COF showed large specific surface area (2057 m 2 g -1 ), good crystal structure, ordered pore size distribution (2.3 nm), stable chemical properties and good reusability. Accordingly, a simple and efficient method based on dispersive solid-phase extraction (d-SPE) and atmospheric pressure gas chromatography tandem mass spectrometry (APGC-MS/MS) was developed for the determination of quinones in complex samples. The established method demonstrated a wide liner range, good linearity (r>0.9990), high enrichment factors (EFs, 24-69-folds) and low detection limits (LODs, 0.200-30.0 pg L -1 , S/N≥3). On this basis, the suggested method was successfully applied to sensitively detect the eight ultratrace quinones in mice plasma. Overall, the established method has provided a powerful tool for the enrichment and detection of ultratrace quinones in complex samples, presenting the promising application of TatDha-COF in sample pretreatment.