Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2.

Yee Ling LauIlyiana IsmailNur Izati MustapaMeng Yee LaiTuan Suhaila Tuan SohAfifah HassanKalaiarasu M PeariasamyYee Leng Lee Yoong Min Chong I-Ching SamPik Pin Goh

Published in: PeerJ (2020)

This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.

Keyphrases

sars cov
loop mediated isothermal amplification
high throughput
respiratory syndrome coronavirus
sensitive detection
transcription factor
single cell