Formalin fixation for optimal concordance of programmed death-ligand 1 immunostaining between cytologic and histologic specimens from patients with non-small cell lung cancer.
Bregje M KoomenJose van der Starre-GaalJudith M VonkJan H von der ThüsenJacqueline J C van der MeijKim MonkhorstStefan M WillemsWim TimensNils A 't HartPublished in: Cancer cytopathology (2020)
Performing PD-L1 staining on cytologic specimens fixed in alcohol-based fixatives could result in false-negative immunostaining results, whereas fixation in formalin leads to higher and more histology-concordant PD-L1 immunostaining. The deleterious effect of alcohol fixation could be reversed to some degree by postfixation in formalin.
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