LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz.
Katarzyna SzewczykWioleta PietrzakKatarzyna KlimekAnna Grzywa-CelińskaRafał CelińskiMarek GogaczPublished in: Molecules (Basel, Switzerland) (2022)
Liquid chromatography electrospray ionization tandem mass spectrometric (LC-ESI-MS/MS) qualitative and quantitative analysis of different extracts from the aerial parts and roots of Alchemilla acutiloba led to the identification of phenolic acids and flavonoids. To the best of our knowledge, isorhamnetin 3-glucoside, kaempferol 3-rutinoside, narcissoside, naringenin 7-glucoside, 3- O -methylquercetin, naringenin, eriodictyol, rhamnetin, and isorhamnetin were described for the first time in Alchemilla genus. In addition, the antioxidant, anti-inflammatory and cytotoxic activity of all extracts were evaluated. The results clearly showed that among analyzed extracts, the butanol extract of the aerial parts exhibited the highest biological activity comparable with the positive controls used.
Keyphrases
- ms ms
- anti inflammatory
- liquid chromatography
- mass spectrometry
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- oxidative stress
- tandem mass spectrometry
- healthcare
- high resolution mass spectrometry
- ultra high performance liquid chromatography
- high performance liquid chromatography
- high resolution
- bioinformatics analysis
- solid phase extraction
- gas chromatography