16 α -[(18)F]-fluoroestradiol ([(18)F]FES), a steroid-based positron emission tomography (PET) tracer, has emerged as a dependable tracer for the evaluation and management of estrogen receptor-positive (ER+) breast cancer patients. We have developed a fully automatic, one-pot procedure for the synthesis of [(18)F]FES using the Eckert & Ziegler (E & Z) radiomodular system. After [(18)F]fluorination, the intermediate was hydrolyzed with 2.0 M HCl twice and neutralized with sodium bicarbonate. After high-performance liquid chromatography (HPLC) purification, the decay-corrected radiochemical yield and purity of [(18)F]FES were 40 ± 5.0% (n = 12) and >97%, respectively. The product was stable up to 10 h. Total synthesis time including HPLC purification was 80 min. This new, fully automated rapid synthetic procedure provided high and reproducible yields of [(18)F]FES. Quality control (QC) tests showed that the [(18)F]FES produced by this method met all specifications for human injection.
Keyphrases
- estrogen receptor
- positron emission tomography
- high performance liquid chromatography
- computed tomography
- simultaneous determination
- tandem mass spectrometry
- pet imaging
- solid phase extraction
- quality control
- ms ms
- mass spectrometry
- pet ct
- machine learning
- deep learning
- endothelial cells
- minimally invasive
- liquid chromatography
- cancer therapy
- adverse drug
- high throughput
- drug delivery
- tyrosine kinase
- loop mediated isothermal amplification
- sensitive detection
- high resolution
- electronic health record