Physiochemical and bactericidal activity evaluation: Silver-augmented 3D-printed scaffolds-An in vitro study.
Vasudev Vivekanand NayakNick TovarJacques Henri HacquebordSimone DuarteBeatriz Helena Dias PanarielloCaroline Coradi TononPablo J AtriaPaulo G CoelhoLukasz WitekPublished in: Journal of biomedical materials research. Part B, Applied biomaterials (2021)
XRD, FTIR, ICP-MS, TGA, and DSC confirmed presence of Ag and PO4 groups, whereas electron microscopy showed a decrease in Ca and an increase in Ag ions, decreasing Ca/P ratio with increasing surfactant concentrations. PrestoBlue assays yielded an increase in fluorescence cell counts among experimental groups with lower concentrations of Ag characterized by their characteristic trapezoidal shape whereas cytotoxicity was observed at higher concentrations. Similar observations were made with alkaline phosphatase assays. Antimicrobial evaluation showed reduced colony-forming units (CFU) among all experimental groups when compared to 100% β-TCP. β-TCP scaffolds augmented with Ag ions facilitate antibacterial effects while promoting osteoblast adhesion and proliferation.
Keyphrases
- quantum dots
- visible light
- electron microscopy
- highly efficient
- energy transfer
- high throughput
- tissue engineering
- multiple sclerosis
- single cell
- staphylococcus aureus
- signaling pathway
- silver nanoparticles
- ms ms
- single molecule
- virtual reality
- escherichia coli
- cystic fibrosis
- mesenchymal stem cells
- aqueous solution
- biofilm formation
- bone regeneration