Statistical optimization and characterization of monodisperse and stable biogenic gold nanoparticle synthesis using Streptomyces sp. M137-2.

Microbial synthesis of gold nanoparticles (AuNPs), which are used in various forms with different properties in medicine, as a renewable bioresource has become increasingly important in recent years. In this study, statistical optimization of stable and monodispersed AuNPs synthesis was performed using a cell-free fermentation broth of Streptomyces sp. M137-2 and AuNPs were characterized, and their cytotoxicity was determined. The three factors determined as pH, gold salt (HAuCl 4 ) concentration, and incubation time, which are effective in the extracellular synthesis of biogenic AuNPs, were optimized by Central Composite Design (CCD) and then UV-Vis Spectroscopy, Dynamic Light Scattering (DLS), X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), Scanning Transmission Electron Microscope (STEM), size distribution, Fourier-Transform Infrared (FT-IR) Spectroscopy, X-Ray Photoelectron Spectrophotometer (XPS) and stability analyzes of AuNPs were carried out. Optimum values of the factors were determined as pH 8, 10 - 3  M HAuCl 4 , and 72 h incubation using Response Surface Methodology (RSM). Almost spherical AuNPs with 20-25 nm protein corona on the surface, 40-50 nm in size, monodisperse, and highly stable form were synthesized. Biogenic AuNPs were confirmed from characteristic diffraction peaks in the XRD pattern, UV-vis peak centred at 541 nm. The FT-IR results confirmed the role of Streptomyces sp. M137-2 metabolites in the reduction and stabilization of AuNPs. The cytotoxicity results also showed that AuNPs obtained using Streptomyces sp. can be used safely in medicine. This is the first report to perform statistical optimization of size-dependent biogenic AuNPs synthesis using a microorganism.