Size Attenuated Copper Doped Zirconia Nanoparticles Enhances In Vitro Antimicrobial Properties.
S NishakavyaAgnishwar GirigoswamiA GopikrishnaR DeepaA DivyaS AjithKoyeli GirigoswamiPublished in: Applied biochemistry and biotechnology (2022)
Biofilm formation hinders the activity of antimicrobial drugs at the site of infections and any agent that can act on both Gram-positive and Gram-negative bacteria by inhibiting the bacterial growth and rupturing the biofilm is needed to manage infection. In the present study, we have synthesized zirconia nanoparticles (ZrO 2 NPs) and copper doped zirconia nanoparticles (Cu-ZrO 2 NPs) and characterized them using dynamic light scattering, X-ray diffractometry, and scanning electron microscopy (SEM). The size of the Cu-ZrO 2 NPs drastically reduced compared to ZrO 2 NPs, and the antimicrobial activity was studied against Gram-positive bacteria (Lactobacillus sp.) and Gram-negative bacteria (Pseudomonas aeruginosa), respectively. The synthesized Cu-ZrO 2 NPs showed superior inhibitory action against Lactobacillus sp. compared to ZrO 2 NPs, due to the negatively charged cell wall of Lactobacillus sp., which could attract readily the positively charged Cu-ZrO 2 NPs, thereby inhibiting its activity. The biocompatibility was tested using XTT assay in FL cells, and the results demonstrated that Cu-ZrO 2 NPs were nontoxic to mammalian cells. Hence, it could be proposed that the synthesized Cu-ZrO 2 NPs possess possible biomedical applications and can be used as antibacterial agents without causing toxicity in mammalian cells.
Keyphrases
- oxide nanoparticles
- pseudomonas aeruginosa
- biofilm formation
- staphylococcus aureus
- electron microscopy
- metal organic framework
- aqueous solution
- induced apoptosis
- cystic fibrosis
- cell wall
- high resolution
- escherichia coli
- signaling pathway
- gram negative
- oxidative stress
- magnetic resonance
- acinetobacter baumannii
- walled carbon nanotubes
- single cell
- pi k akt
- cell cycle arrest