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Size Attenuated Copper Doped Zirconia Nanoparticles Enhances In Vitro Antimicrobial Properties.

S NishakavyaAgnishwar GirigoswamiA GopikrishnaR DeepaA DivyaS AjithKoyeli Girigoswami
Published in: Applied biochemistry and biotechnology (2022)
Biofilm formation hinders the activity of antimicrobial drugs at the site of infections and any agent that can act on both Gram-positive and Gram-negative bacteria by inhibiting the bacterial growth and rupturing the biofilm is needed to manage infection. In the present study, we have synthesized zirconia nanoparticles (ZrO 2 NPs) and copper doped zirconia nanoparticles (Cu-ZrO 2 NPs) and characterized them using dynamic light scattering, X-ray diffractometry, and scanning electron microscopy (SEM). The size of the Cu-ZrO 2 NPs drastically reduced compared to ZrO 2 NPs, and the antimicrobial activity was studied against Gram-positive bacteria (Lactobacillus sp.) and Gram-negative bacteria (Pseudomonas aeruginosa), respectively. The synthesized Cu-ZrO 2 NPs showed superior inhibitory action against Lactobacillus sp. compared to ZrO 2 NPs, due to the negatively charged cell wall of Lactobacillus sp., which could attract readily the positively charged Cu-ZrO 2 NPs, thereby inhibiting its activity. The biocompatibility was tested using XTT assay in FL cells, and the results demonstrated that Cu-ZrO 2 NPs were nontoxic to mammalian cells. Hence, it could be proposed that the synthesized Cu-ZrO 2 NPs possess possible biomedical applications and can be used as antibacterial agents without causing toxicity in mammalian cells.
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