Single-Layered Microfluidic Network-Based Combinatorial Dilution for Standard Simplex Lattice Design.
Kangsun LeeChoong KimKwang W OhPublished in: Micromachines (2018)
In this paper, we presented a straightforward strategy to generate 15 combinations of three samples based on an experimental simplex lattice design using a single-layer microfluidic network. First, we investigated the performances of the plain structural and the groove structural combinatorial devices by computational simulation (CFD-ACE+). The simulated output concentrations were extremely close to the desirable values within an absolute error of less than 1%. Based on the simulated designs, polydimethylsiloxane (PDMS) devices were fabricated with soft lithography and tested with fluorescent dye (sodium salt). The mixing results for 15 combinations showed good performance, with an absolute error of less than 4%. We also investigated two liquid handling methods (bottom⁻up and top⁻down) for high-throughput screening and assay. The liquid-handling methods were successfully accomplished by adding the systematic structured groove sets on the mixing channels.
Keyphrases
- high throughput
- circulating tumor cells
- single cell
- label free
- atomic force microscopy
- ionic liquid
- highly efficient
- quantum dots
- angiotensin ii
- living cells
- reduced graphene oxide
- angiotensin converting enzyme
- liquid chromatography
- mass spectrometry
- high speed
- high resolution
- single molecule
- network analysis
- fluorescent probe
- ms ms
- simultaneous determination
- transition metal
- tandem mass spectrometry