Functional requirement of the Arabidopsis importin-α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1.
Daniel LüdkeCharlotte RothSieglinde A KamradJana MesserschmidtDenise HartkenJonas AppelBojan F HörnichQiqi YanStefan KuschMelanie KlenkeAnnette GunkelLennart WirthmuellerMarcel WiermerPublished in: The Plant journal : for cell and molecular biology (2020)
IMPORTIN-α3/MOS6 (MODIFIER OF SNC1, 6) is one of nine importin-α isoforms in Arabidopsis that recruit nuclear localization signal-containing cargo proteins to the nuclear import machinery. IMP-α3/MOS6 is required genetically for full autoimmunity of the nucleotide-binding leucine-rich repeat immune receptor mutant snc1 (suppressor of npr1-1, constitutive 1) and MOS6 also contributes to basal disease resistance. Here, we investigated the contribution of the other importin-α genes to both types of immune responses, and we analyzed potential interactions of all importin-α isoforms with SNC1. By using reverse-genetic analyses in Arabidopsis and protein-protein interaction assays in Nicotiana benthamiana, we provide evidence that among the nine α-importins in Arabidopsis, IMP-α3/MOS6 is the main nuclear transport receptor of SNC1, and that IMP-α3/MOS6 is required selectively for autoimmunity of snc1 and basal resistance to mildly virulent Pseudomonas syringae in Arabidopsis.
Keyphrases
- transcription factor
- quantum dots
- protein protein
- room temperature
- plant growth
- reduced graphene oxide
- cell wall
- immune response
- small molecule
- transition metal
- binding protein
- visible light
- highly efficient
- dna binding
- genome wide
- high throughput
- genome wide identification
- celiac disease
- gold nanoparticles
- dendritic cells
- amino acid
- toll like receptor
- biofilm formation
- risk assessment
- single cell
- climate change
- atomic force microscopy
- single molecule