A sustained-release PDGF-BB nanocomposite hydrogel for DM-associated bone regeneration.
Yaxin LiZiyang LiuChen ZhaoChenci XuAiri ShinJiaqing WuDejian LiKailin LinJiaqiang LiuPublished in: Journal of materials chemistry. B (2023)
Regeneration of bone tissue in the environment of diabetes mellitus (DM) remains one of the clinical challenges, with malfunction of stem cells in a high-glucose microenvironment being the primary obstacle. We designed an injectable sustained-release PDGF-BB nanocomposite hydrogel. PDGF-BB, a star molecule for treating various complications of DM, was used for the first time for DM-associated bone regeneration, and we showed that it restored stem cell proliferation and migration and facilitated osteogenesis inhibition under high glucose stimulation by activating ERK and AKT pathways. To address the requirements for continuous PDGF-BB release in GelMA while also increasing mechanical strength, nanoclay LAPONITE® was added, which may still exhibit pro-osteogenic activity in diabetic environments by releasing bioactive ions (Si 4+ , Mg 2+ , and Li + ). This injectable hydrogel heals calvarial lesions successfully in diabetic rats and has the potential to be used as a direct and effective tool for treating diabetic patients.
Keyphrases
- bone regeneration
- stem cells
- high glucose
- hyaluronic acid
- growth factor
- tissue engineering
- endothelial cells
- smooth muscle
- wound healing
- vascular smooth muscle cells
- diabetic rats
- signaling pathway
- drug delivery
- glycemic control
- recombinant human
- quantum dots
- oxidative stress
- type diabetes
- cell proliferation
- reduced graphene oxide
- cell therapy
- bone marrow
- angiotensin ii
- pi k akt
- mesenchymal stem cells
- room temperature
- carbon nanotubes
- solid phase extraction
- metabolic syndrome
- mass spectrometry
- visible light
- postmenopausal women
- anti inflammatory
- human health
- skeletal muscle
- insulin resistance